Manual  

 

   
bulletDownloading necessary files
bulletConstructing Gene Information File
bulletConstructing Gene Ontology Structure File
bulletGene Ontology Related Calculations
bulletArray Background
bulletThe number of probe sets associated with every GO term
bulletThe number of GO terms associated with every probe set
bulletTracing GO
bulletRetrieving Probe Sets for Homologous Genes

        

 

 

 

 

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  A short tutorial

Downloading necessary files

Please see the download page for instructions. We no longer support the "automatic download" function for NetAffx csv files.

Constructing Gene Information File

A Gene Information File is a tabular text file, annotating all the probe sets of a given Affymetrix array type. Each line of it contains the annotation information of a probe set, including Genbank ID, LocusLink ID, gene name, GO terms, protein domain terms, signaling pathways, cytoband and Affymetrix descriptions. The table below shows how a Gene Information File looks like.

Probe Set

Genbank

LocusLink

Name

Gene Ontology

Protein Domain

Pathway

Chrom.

Desc.

100001_at

M18228

12502

CD3 antigen, gamma polypeptide

|7166|7165|

|3598|3110|

 

|9|

100002_at

X70393

16426

inter-alpha trypsin inhibitor, heavy chain 3

|4867|4866|

|1117|2035|

 

|14|

100016_at

Z12604

17385

Matrix metalloproteinase11

|6805|9410|

|6026|1818|

|Matrix Metalloproteinases|

|10|

100017_at

U68267

53311

myosin binding protein H

|7517|9887|

|3598|3961|

|Cytoskeletal Pathway|

|1|

Click on Menu "Annotation => Gene Information File", and the following dialog will show up.

Clicking on the boxes for file names, the user is allowed to choose the file names of a NetAffx file and a GO file from its computer. These files are obtained by Accessing Online Databases.

 

Special note: For the GO structure file to be acceptable by GoSurfer, please use Excel to re-order its rows so that the second column is in ascending alphabetical order.  

 

This page is under construction.

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This site was last updated 11/15/04